C-terminal His-tagged Calcitonin (5 grams) and untagged GLP-1 peptide (10 grams) are accidentally mixed in 1L of 0.5M imidazole buffer.Marketing Research and Data Analysis QC confirmed that there are no other impurities in the mixture. Design a suitable downstream purification scheme to obtain pharmaceutical-grade calcitonin and GLP-1, each in lyophilized form.
Please include the following in your report:
- Process flow diagram
- Stream table data
- Technical drawing and specification sheet of at least 3 selected unit operations
- Design calculations to prove the purification will operate as intended
- Other aspects selected based on their functioning in the unit