Abstract: We started by performing PCR purification, rapid ligation, andHuman Computer Interaction transformation experiments. The reason we performed this experiment is to remove the active thermophilic DNA polymerase present in the PCR mixture, catalyze the formation of phosphodiester presence of ATP between double-stranded DNA with 3′ hydroxyl and 5′ phosphate termini, and to transform the Escherichia coli bacteria when expose it to extracellular plasmid DNA that contains the pGreen gene and the gene for ampicillin resistance. In this experiment, we got 48.1 for the DNA concentration and 1.82 for the purity. Next, we performed white/blue screening and observed a pGreen transformation. The purpose of the experiment is to perform a white/blue screening plate from the previous experiment along with preparing and viewing slides of transformed E. coli with the pGreen plasmid under the fluorescence microscope. But unfortunately the culture/ plates were contaminated from the previous lab so we just used the plates that had the control. Then, we did a plasmid extraction and restriction enzyme digestion lab and the purpose was to cut the plasmid into specific sized pieces and analyze the resulting fragments by gel electrophoresis. For the DNA concentration we got 46.7 and the purity was 2.05. After that we performed a soil DNA extraction and gel electrophoresis. The main purpose for DNA extraction in general was to study the genetic causes of disease and for the development of diagnostics and drugs, sequencing genomes, and detecting bacteria and viruses. The DNA concentration was 22.3 ng/uL and the purity was 1.92. Finally, we performed a Bioinformatics analysis of 16s rRNA genes to get results for BLAST identify a strain using the EzTaxon
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