Question 1
Using the OMIM database information for Type 2 Diabetes Mellitus (T2D)
(https://omim.org/entry/125853), solve the following tasks.
Question 1a
Extract out the genes associated with T2D and use the list as input in the online tool DAVID (Database for Annotation, Visualization and Integrated Discovery).
(5 marks)
Question 1b
Create a functional annotation clustering of the gene list and paste screenshots of the results from GOTERM_BP/CC/MF_DIRECT and KEGG_PATHWAY. Report the clusters having enrichment score above 1.
(5 marks)
Question 1c
Appraise the results obtained and discuss the relevancy of the findings to the gene list.
(5 marks)
Question 2
Using the NCBI database, explore the gene with Gene ID: 79923.
Question 2a State the gene symbol given to this ID and the full official name of the gene.
(2 marks)
Question 2b
Discuss the function of this gene in your own words.
(5 marks)
Question 2c
Determine the mRNA transcripts and protein isoforms of this gene.
(5 marks)
Question 2d
Describe FIVE (5) pathways which the corresponding gene is involved in.
(5 marks)
Question 2e
Identify the conserved domains in the protein isoforms of this gene.
(3 marks)