DNA sequencing reactions

147 views 8:58 am 0 Comments July 15, 2023

The following data (top of p. 549) are from a DNaseI footprinting experiment in which either RNA polymerase or a repressorHuman Computer Interaction protein was added to a labeled DNA fragment, and then the complex was digested with DNaseI. DNA sequencing reactions were also performed on the same DNA so the bases that were protected by proteins binding could be identified. (Notice that DNaseI does not cut after each base in the DNA fragment.) a. What is the sequence of the DNA in this fragment? b. Mark on the sequence the region where the repressor binds. c. Mark on the sequence the region where RNA polymerase binds

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